Primary Identifier | MGI:6712697 | Allele Type | Endonuclease-mediated |
Attribute String | Inducible, Inserted expressed sequence | Gene | Gt(ROSA)26Sor |
Strain of Origin | C57BL/6J | Induced With | tamoxifen |
Is Recombinase | false | Is Wild Type | false |
molecularNote | CRISPR/cas9 genome editing is used to insert a CAG promoter followed by a loxP:STOP:loxP (LSL) followed by the ER-Hoxb8 fusion protein into the Gt(ROSA)26Sor locus. Transient transfection with cre recombinase removes the LSL and leaves a single loxP site. The ER-Hoxb8 fusion protein comprises the hormone binding domain of the human estrogen receptor alpha fused to the murine Hoxb8 sequence. In this system, the ER-Hoxb8 protein is constitutively expressed; the estrogen receptor fusion renders the protein functionally dependent on estrogen. |