| First Author | Li S | Year | 2019 |
| Journal | Proc Natl Acad Sci U S A | PubMed ID | 30792349 |
| Mgi Jnum | J:274306 | Mgi Id | MGI:6283126 |
| Doi | 10.1073/pnas.1821111116 | Citation | Li S, et al. (2019) Role for nuclear interleukin-37 in the suppression of innate immunity. Proc Natl Acad Sci U S A |
| abstractText | The IL-1 family member IL-37 broadly suppresses innate inflammation and acquired immunity. Similar to IL-1alpha and IL-33, IL-37 is a dual-function cytokine in that IL-37 translocates to the nucleus but also transmits a signal via surface membrane receptors. The role of nuclear IL-37 remains unknown on the ability of this cytokine to inhibit innate inflammation. Here, we compared suppression of innate inflammation in transgenic mice expressing native human IL-37 (IL-37Tg) with those of transgenic mice carrying the mutation of aspartic acid (D) to alanine (A) at amino acid 20 (IL-37D20ATg). The mutation D20A prevents cleavage of caspase-1, a step required for IL-37 nuclear translocation. In vitro, peritoneal macrophages from IL-37Tg mice reduced LPS-induced IL-1beta, IL-6, TNFalpha and IFNgamma by 40-50% whereas in macrophages from IL-37D20ATg mice this suppression was not observed, consistent with loss of nuclear function. Compared with macrophages from IL-37Tg mice, significantly less or no suppression of LPS-induced MAP kinase and NFkappaB activation was also observed in macrophages from IL-37D20ATg mice. In vivo, levels of IL-1beta, IL-6, and TNFalpha in the lungs and liver were markedly reduced during endotoxemia in IL-37Tg mice but not observed in IL-37D20ATg mice. However, suppression of innate inflammation remains intact in the IL-37D20A mice once the cytokine is released from the cell and binds to its receptor. These studies reveal a nuclear function for suppression of innate inflammation and are consistent with the dual function of IL-37 and a role for caspase-1 in limiting inflammation. |
