Primary Identifier | MGI:7428755 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Slc39a5 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | An FRT-flanked PGK-neomycin selection cassette and a loxP site were inserted upstream of exon 4 and a second loxP site was inserted downstream of exon 4. The neomycin selection cassette was removed via flp-mediated recombination. Exon 4 was deleted via cre-mediated recombination. Western blot analysis confirmed absence of protein in islets. |