| First Author | Miosge N | Year | 2000 |
| Journal | J Histochem Cytochem | Volume | 48 |
| Issue | 2 | Pages | 229-38 |
| PubMed ID | 10639489 | Mgi Jnum | J:66122 |
| Mgi Id | MGI:1928005 | Doi | 10.1177/002215540004800208 |
| Citation | Miosge N, et al. (2000) Nidogen-1. Expression and ultrastructural localization during the onset of mesoderm formation in the early mouse embryo. J Histochem Cytochem 48(2):229-38 |
| abstractText | Nidogen-1, a key component of basement membranes, is considered to function as a link between laminin and collagen Type IV networks and is expressed by mesenchymal cells during embryonic and fetal development. It is not clear which cells produce nidogen-1 in early developmental stages when no mesenchyme is present. We therefore localized nidogen-1 and its corresponding mRNA at the light and electron microscopic level in Day 7 mouse embryos during the onset of mesoderm formation by in situ hybridization, light microscopic immunostaining, and immunogold histochemistry. Nidogen-1 mRNA was found not only in the cells of the ectoderm-derived mesoderm but also in the cytoplasm of the endoderm and ectoderm, indicating that all three germ layers express it. Nidogen-1 was localized only in fully developed basement membranes of the ectoderm and was not seen in the developing endodermal basement membrane or in membranes disrupted during mesoderm formation. In contrast, laminin-1 and collagen Type IV were present in all basement membrane types at this developmental stage. The results indicate that, in the early embryo, nidogen-1 may be expressed by epithelial and mesenchymal cells, that both cell types contribute to embryonic basement membrane formation, and that nidogen-1 might serve to stabilize basement membranes in vivo. (J Histochem Cytochem 48:229-237, 2000) |
