Primary Identifier | MGI:7388303 | Allele Type | Targeted |
Attribute String | Modified regulatory region | Gene | Rr149350 |
Transmission | Germline | Strain of Origin | 129S4/SvJae |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The 3kb KpnI-AccI restriction sequence containing DNAse1 hypersensitivity sites 9-11 upstream of Trbd1 which form or are part of its promoter/enhancer, was replaced with a loxP site flanked neomycin resistance gene cassette, sequence for prearranged segments Trbd1/Trbj2-5 and a third loxP site. The neo cassette and the inserted gene segments were removed through subsequent cre-mediated recombination. |