Primary Identifier | MGI:6294146 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Klb |
Inheritance Mode | Not Specified | Strain of Origin | C57BL/6NJ |
Is Recombinase | false | Is Wild Type | false |
Project Collection | IMPC |
molecularNote | This allele was generated at The Jackson Laboratory by electroporating Cas9 protein and 2 guide sequences TAGTTCGTTTACAAAGTGCC and GCTGAATTAACCACGCACCA, which resulted in a 369 bp deletion beginning at Chromosome 5 position 65,375,614 bp and ending after 65,375,982 bp (GRCm38/mm10). This mutation deletes ENSMUSE00000186798 (exon 3) and 100 bp of flanking intronic sequence including the splice acceptor and donor and is predicted to cause a change of amino acid sequence after residue 444 and early truncation 50 amino acids later. |