| First Author | Emrich F | Year | 2019 |
| Journal | J Cell Mol Med | Volume | 23 |
| Issue | 10 | Pages | 7000-7009 |
| PubMed ID | 31402541 | Mgi Jnum | J:299094 |
| Mgi Id | MGI:6490121 | Doi | 10.1111/jcmm.14587 |
| Citation | Emrich F, et al. (2019) Anatomically specific reactive oxygen species production participates in Marfan syndrome aneurysm formation. J Cell Mol Med 23(10):7000-7009 |
| abstractText | Marfan syndrome (MFS) is a connective tissue disorder that results in aortic root aneurysm formation. Reactive oxygen species (ROS) seem to play a role in aortic wall remodelling in MFS, although the mechanism remains unknown. MFS Fbn1(C1039G/+) mouse root/ascending (AS) and descending (DES) aortic samples were examined using DHE staining, lucigenin-enhanced chemiluminescence (LGCL), Verhoeff's elastin-Van Gieson staining (elastin breakdown) and in situ zymography for protease activity. Fbn1(C1039G/+) AS- or DES-derived smooth muscle cells (SMC) were treated with anti-TGF-beta antibody, angiotensin II (AngII), anti-TGF-beta antibody + AngII, or isotype control. ROS were detected during early aneurysm formation in the Fbn1(C1039G/+) AS aorta, but absent in normal-sized DES aorta. Fbn1(C1039G/+) mice treated with the unspecific NADPH oxidase inhibitor, apocynin reduced AS aneurysm formation, with attenuated elastin fragmentation. In situ zymography revealed apocynin treatment decreased protease activity. In vitro SMC studies showed Fbn1(C1039G/+) -derived AS SMC had increased NADPH activity compared to DES-derived SMC. AS SMC NADPH activity increased with AngII treatment and appeared TGF-beta dependent. In conclusion, ROS play a role in MFS aneurysm development and correspond anatomically with aneurysmal aortic segments. ROS inhibition via apocynin treatment attenuates MFS aneurysm progression. AngII enhances ROS production in MFS AS SMCs and is likely TGF-beta dependent. |
