Primary Identifier | MGI:5824224 | Allele Type | Targeted |
Attribute String | Epitope tag | Gene | Runx2 |
Transmission | Germline | Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting vector contains a loxP-flanked PGKneo cassette downstream of Biotin-3xFLAG (BioFL) sequence (encoding a biotin recognition motif and 3 copies of the FLAG epitope) fused to the C-terminus of the gene. Cre-mediated recombination removed the floxed neo cassette. Western blotting detected levels of Rnx2-BioFL protein similar to wild-type. |