| First Author | Ritter B | Year | 1999 |
| Journal | FEBS Lett | Volume | 454 |
| Issue | 3 | Pages | 356-62 |
| PubMed ID | 10431838 | Mgi Jnum | J:56265 |
| Mgi Id | MGI:1340716 | Doi | 10.1016/s0014-5793(99)00830-3 |
| Citation | Ritter B, et al. (1999) PACSIN 2, a novel member of the PACSIN family of cytoplasmic adapter proteins. FEBS Lett 454(3):356-62 |
| abstractText | The PACSIN-related proteins are cytoplasmic adapter proteins with a common arrangement of domains and conserved regions. Here we report the cloning, sequencing, and expression of PACSIN 2, a novel member of the PACSIN protein family and accordingly rename the original PACSIN to PACSIN 1. The sequences of the murine and human cDNAs reveal an open reading frame encoding a putative protein of 486 residues. Despite its high sequence similarity to PACSIN 1, PACSIN 2 is encoded by distinct transcripts in human and mouse, in particular displaying a ubiquitous expression pattern. Immunofluorescence microscopy of PACSIN 2-transfected NIH3T3 fibroblasts reveal a broad, vesicle-like cytoplasmic staining. In contrast to FAP52, another PACSIN-related protein derived from chicken brain, PACSIN 2 could not be detected at focal contacts. Taken together, these findings suggest that PACSIN 2 is a novel PACSIN isoform with similar domain and motif arrangement, but an unrestricted expression pattern, which may participate in the organization of the actin cytoskeleton and the regulation of vesicular traffic. |
