| First Author | Hardas BD | Year | 1994 |
| Journal | Genomics | Volume | 21 |
| Issue | 2 | Pages | 359-63 |
| PubMed ID | 8088830 | Mgi Jnum | J:18468 |
| Mgi Id | MGI:66750 | Doi | 10.1006/geno.1994.1277 |
| Citation | Hardas BD, et al. (1994) Direct evidence for homologous sequences on the paracentric regions of human chromosome 1. Genomics 21(2):359-63 |
| abstractText | Calcyclin is a member of the S100 family of proteins, many of which are encoded by genes that have been localized to the proximal long arm of human chromosome 1 (bands q21-q22). A 450-kb yeast artificial chromosome clone containing the human calcyclin gene was identified by PCR screening and used as a probe for fluorescence in situ hybridization (FISH). Along with the expected hybridization to 1q21, simultaneous, specific hybridization to the centromeric region of the short arm of chromosome 1 was also observed. An identical pattern of hybridization was observed when microdissected 1q21 DNA sequences were used as a probe for FISH, confirming the presence of homologous sequences flanking both sides of the centromere of human chromosome 1. These results are consistent with a model in which human chromosome 1 arose by insertion of the centromere and heterochromatin into an ancestral chromosome containing chromosome-specific repetitive sequences. |
