| First Author | Opdecamp K | Year | 1992 |
| Journal | Nucleic Acids Res | Volume | 20 |
| Issue | 2 | Pages | 171-8 |
| PubMed ID | 1371343 | Mgi Jnum | J:750 |
| Mgi Id | MGI:49284 | Doi | 10.1093/nar/20.2.171 |
| Citation | Opdecamp K, et al. (1992) Methylation of an alpha-foetoprotein gene intragenic site modulates gene activity. Nucleic Acids Res 20(2):171-8 |
| abstractText | By comparing the methylation pattern of Mspl/Hpall sites in the 5' region of the mouse alpha-foetoprotein (AFP) gene of different cells (hepatoma cells, foetal and adult liver, fibroblasts), we found a correlation between gene expression and unmethylation of a site located in the first intron of the gene. Other sites did not show this correlation. In transfection experiments of unmethylated and methylated AFP-CAT chimeric constructions, we then showed that methylation of the intronic site negatively modulates expression of CAT activity. We also found that a DNA segment centered on this site binds nuclear proteins; however methylation did not affect protein binding. |
