| First Author | Sakurai T | Year | 2021 |
| Journal | STAR Protoc | Volume | 2 |
| Issue | 2 | Pages | 100509 |
| PubMed ID | 34027476 | Mgi Jnum | J:347386 |
| Mgi Id | MGI:7622075 | Doi | 10.1016/j.xpro.2021.100509 |
| Citation | Sakurai T, et al. (2021) Production of single- and multiple-gene-modified mice via maternal SpCas9-based gene editing. STAR Protoc 2(2):100509 |
| abstractText | Maternally and transiently accumulated SpCas9 (maternal SpCas9) in a zygote derived from a systemically SpCas9-expressing transgenic mouse strain was used to generate single- and multiple-gene-modified mice. Maternal SpCas9-based gene editing allows for high indel and knockin mutation efficiency, low mosaicism, increased pup delivery rate, and simultaneous induction of mutations at multiple loci in contrast to conventional CRISPR/SpCas9-based gene editing. For complete details on the use and execution of this protocol, please refer to Sakurai et al. (2020). |
