Primary Identifier | MGI:5818151 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Begain |
Transmission | Germline | Strain of Origin | C57BL/6N |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site was inserted into intron 3 and an FRT site flanked neomycin resistance gene cassette and a second loxP site into intron 5. Exons 4 and 5 (and the neo cassette) were deleted through cre-mediated recombination, creating a knock-out allele. RT-PCR confirmed absence of transcription from this allele in spinal dorsal horns and dorsal root ganglia. |