First Author | Hemenway CS | Year | 1998 |
Journal | Oncogene | Volume | 16 |
Issue | 19 | Pages | 2541-7 |
PubMed ID | 9627119 | Mgi Jnum | J:62648 |
Mgi Id | MGI:1859418 | Doi | 10.1038/sj.onc.1202042 |
Citation | Hemenway CS, et al. (1998) The Bmi-1 oncoprotein interacts with dinG and MPh2: the role of RING finger domains. Oncogene 16(19):2541-7 |
abstractText | Experimentally-induced mutations in the C3HC4 RING finger domain of the Bmi-1 oncoprotein block its ability to induce lymphomas in mice. In this report, the role of the Bmi-1 RING finger in mediating protein-protein interactions is examined using the yeast two-hybrid system. Bmi-1 interacts directly with the RING finger protein dinG/RING1B. Heterodimerization of the two proteins requires the intact RING finger structures of both Bmi-1 and dinG. Although the RING finger domains are necessary for dimerization, they are not sufficient for this process as residues outside the C3HC4 motif are also required. Thus, binding specificity may be partly conferred by residues outside the RING motif. Both Bmi-1 and dinG interact with the Polyhomeotic protein MPh2 through binding domains apart from the RING finger. The data suggest a model whereby Bmi-1, dinG, and MPh2 form a stable heterotrimeric complex in which each protein contributes to the binding of the others. |