| First Author | Luther RJ | Year | 2012 |
| Journal | Mol Genet Metab | Volume | 106 |
| Issue | 1 | Pages | 62-7 |
| PubMed ID | 22386849 | Mgi Jnum | J:325857 |
| Mgi Id | MGI:6874816 | Doi | 10.1016/j.ymgme.2012.02.009 |
| Citation | Luther RJ, et al. (2012) Acadl-SNP based genotyping assay for long-chain acyl-CoA dehydrogenase deficient mice. Mol Genet Metab 106(1):62-7 |
| abstractText | The long-chain acyl-CoA dehydrogenase (LCAD) (Acadl=gene; LCAD=protein) deficient mouse model has been important in evaluating the role of mitochondrial fatty acid oxidation of long-chain fatty acids in metabolic disorders. The insertion vector-based gene targeting strategy used to generate this model has made it difficult to distinguish homozygous and heterozygous genotypes containing targeted Acadl alleles in LCAD-deficient mice. Herein, we describe the design and validation of Acadl SNP genotyping methods capable of distinguishing between heterozygous and homozygous LCAD-deficient mice. The Acadl SNP genotyping assays are effective at allelic discrimination of both C57BL/6 and 129 mouse strain-based Acadl alleles under conditions including, both low purity and quantity genomic DNA templates. This makes the method practical and provides the necessary tools for genotyping the LCAD-deficient mouse model. |
