| Primary Identifier | MGI:7521969 | Allele Type | Targeted |
| Attribute String | Reporter | Gene | Myf5 |
| Transmission | Germline | Strain of Origin | C57BL/6NTac |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The targeting construct contains the Myf5 open reading frame (ORF), a Kozak sequence to allow protein translation initiation. A T2A self-cleaving peptide, E2Crimson fluorescent reporter sequence, and 3 nuclear localization signals (3xNLS) inserted after exon 3, just upstream of the 3' untranslated region (UTR), of the Myf5 gene. An FRT-flanked puromycin selection cassette was also included after the 3' UTR. Flp-mediated recombination removed the puromycin cassette. |
