| First Author | Clapcott SJ | Year | 2003 |
| Journal | Mamm Genome | Volume | 14 |
| Issue | 8 | Pages | 495-505 |
| PubMed ID | 12925882 | Mgi Jnum | J:84625 |
| Mgi Id | MGI:2668756 | Doi | 10.1007/s00335-002-2258-4 |
| Citation | Clapcott SJ, et al. (2003) Two ENU-induced mutations in Rasgrf1 and early mouse growth retardation. Mamm Genome 14(8):495-505 |
| abstractText | When paternally transmitted, two independent ENU-induced mutations showed reduced whole body wet weight soon after birth. The mutations were mapped to Chromosome 9 (Chr 9) between the markers D9Mit208 and D9Mit215. Their map position and imprinted status suggested that they might alter RAS protein-specific guanine nucleotide releasing factor 1 expression. Both mutations introduced premature chain termination codons into the coding sequence of Rasgrf1, and no Ras-GRF1 protein was detected in the brain. The GENA53 line had a C to T transition at nucleotide 2137, and the line GENA37 had a T to A transversion at nucleotide 3552 of the cDNA sequence. Mutant mice had near normal body weight at birth, but their weight started to lag behind that of wild-type littermates during the first week, and they were about 15% lighter as adults. |
