| Primary Identifier | MGI:5789261 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Rtl4 |
| Transmission | Germline | Strain of Origin | (C57BL/6NCrlj x CBA/JNCrlj)F1 |
| Is Recombinase | false | Is Wild Type | false |
| molecularNote | The open reading frame was replaced with a frt-flanked neo cassette and loxP sites flanking the open reading frame in exon 7. The floxed exon 7 region was removed via cre-mediated recombination and the neo cassette via flp-mediated recombination. Lack of the open reading frame was confirmed by RT-PCR, however RT-PCR of the 3-UTR (without the ORF) exhibits an approximate 1.5-fold increase in transcript levels. |
