Primary Identifier | MGI:6721057 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Spats1 |
Strain of Origin | (C57BL/6 x DBA/2)F1 | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/Cas9 technology generated a 2 bp deletion (CC) in exon 3, resulting in a frameshift at residue 90 which putatively should generate an aberrant protein of 111 amino acids instead of 269-residue protein. Western blot analysis with an antibody against the central region of the protein did not detect protein in homozygous mice. |