| First Author | Megeney LA | Year | 1996 |
| Journal | Genes Dev | Volume | 10 |
| Issue | 10 | Pages | 1173-83 |
| PubMed ID | 8675005 | Mgi Jnum | J:33260 |
| Mgi Id | MGI:80740 | Doi | 10.1101/gad.10.10.1173 |
| Citation | Megeney LA, et al. (1996) MyoD is required for myogenic stem cell function in adult skeletal muscle. Genes Dev 10(10):1173-83 |
| abstractText | To investigate the function of MyoD in adult skeletal muscle, we interbred MyoD mutant mice with mdx mice, a model for Duchenne and Becker muscular dystrophy. Mice lacking both MyoD and dystrophin displayed a marked increase in severity of myopathy leading to premature death, suggesting a role for MyoD in muscle regeneration. Examination of MyoD mutant muscle revealed elevated numbers of myogenic cells; however, myoblasts derived from these cells displayed normal differentiation potential in vitro. Following injury, MyoD mutant muscle was severely deficient in regenerative ability, and we observed a striking reduction in the in vivo proliferation of myogenic cells during regeneration. Therefore, we propose that the failure of MyoD-deficient muscle to regenerate efficiently is not caused by a reduction in numbers of satellite cells, the stem cells of adult skeletal muscle, but results from an increased propensity for stem-cell self-renewal rather than progression through the myogenic program. |
