| First Author | Chen VP | Year | 2015 |
| Journal | Biochem Pharmacol | Volume | 98 |
| Issue | 4 | Pages | 732-9 |
| PubMed ID | 26514871 | Mgi Jnum | J:228755 |
| Mgi Id | MGI:5708794 | Doi | 10.1016/j.bcp.2015.10.016 |
| Citation | Chen VP, et al. (2015) Radiometric assay of ghrelin hydrolase activity and (3)H-ghrelin distribution into mouse tissues. Biochem Pharmacol 98(4):732-9 |
| abstractText | A high-throughput radiometric assay was developed to characterize enzymatic hydrolysis of ghrelin and to track the peptide's fate in vivo. The assay is based on solvent partitioning of [(3)H]-octanoic acid liberated from [(3)H]-octanoyl ghrelin during enzymatic hydrolysis. This simple and cost-effective method facilitates kinetic analysis of ghrelin hydrolase activity of native and mutated butyrylcholinesterases or carboxylesterases from multiple species. In addition, the assay's high sensitivity facilitates ready evaluation of ghrelin's pharmacokinetics and tissue distribution in mice after i.v. bolus administration of radiolabeled peptide. |
