Primary Identifier | MGI:3775079 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Phex |
Transmission | Germline | Strain of Origin | (129X1/SvJ x 129S1/Sv)F1-Kitl<+> |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 17 was flanked with a loxP site and a floxed neo cassette via homologous recombination. The neo cassette and exon 17 were subsequently removed via in vivo Cre-mediated recombination. This results in a frameshift, premature termination codon and truncated protein. PCR analysis confirmed the presence of a 0.4 kb truncated transcript in mice carrying this allele. |