| First Author | Chuong CM | Year | 1982 |
| Journal | Proc Natl Acad Sci U S A | Volume | 79 |
| Issue | 13 | Pages | 4234-8 |
| PubMed ID | 7051008 | Mgi Jnum | J:37745 |
| Mgi Id | MGI:85132 | Doi | 10.1073/pnas.79.13.4234 |
| Citation | Chuong CM, et al. (1982) Neural cell adhesion molecules in rodent brains isolated by monoclonal antibodies with cross-species reactivity. Proc Natl Acad Sci U S A 79(13):4234-8 |
| abstractText | Previous studies in this laboratory have led to the identification and purification of a chicken cell surface protein named neural cell adhesion molecule (N-CAM) that is involved in neural cell-cell and neurite-neurite interactions. In the present investigation, we have found that a similar molecule exists in the mouse and have confirmed that it is also present in rat neural tissue. A monoclonal antibody to chicken N-CAM that crossreacted with mouse and rat brains and an independently derived monoclonal antibody to mouse N-CAM were used to purify an antigen from perinatal mouse and rat brains. The purified neural antigen resembles chicken N-CAM in its ability to neutralize antibodies that inhibit neural cell aggregation and also in its biochemical properties including molecular weight, sialic acid content, amino acid composition, and autoconversion to a smaller polypeptide. Like chicken N-CAM, the murine molecule is found throughout the nervous system and over the entire neuronal cell surface. These results strongly suggest that the molecule is evolutionarily related to chicken N-CAM and prompt the hypothesis that cell adhesion involving N-CAM is a fundamental mechanism existing in nervous systems of different phylogenetic classes of animals. |
