| First Author | Frankfort BJ | Year | 1995 |
| Journal | Biochem Biophys Res Commun | Volume | 206 |
| Issue | 3 | Pages | 916-26 |
| PubMed ID | 7832805 | Mgi Jnum | J:22534 |
| Mgi Id | MGI:70397 | Doi | 10.1006/bbrc.1995.1130 |
| Citation | Frankfort BJ, et al. (1995) Identification of novel cellular genes transcriptionally suppressed by v-src. Biochem Biophys Res Commun 206(3):916-26 |
| abstractText | Our aim is to identify cellular genes whose transcriptional suppression by the v-src oncogene contributes directly and specifically to the transformed and tumorigenic phenotype. We used a modified PCR-based subtractive hybridization technique to isolate 9 cDNAs whose abundance in NIH3T3 fibroblasts is decreased 3-15-fold following transformation by the activated oncogene, v-src. Sequence analysis reveals that 3 cDNAs are unlike those in GenBank. The remaining 6 cDNAs are indentical or highly similar to rat helix-destabilizing protein gene (hnRNP A1), mouse CTLA-2 alpha cysteine protease, rat cytochrome c oxidase (COX) VIc subunit, mouse Type I collagen, human gravin and a partial human cDNA (clone A7C09) isolated by random automated sequencing. Northern blot analysis indicates that the basal level of transcripts in untransformed NIH3T3 of all the genes except mouse Type I collagen was at least 10-fold lower than that of HMG Co-A Reductase, which is abundantly transcribed. These data suggest that the down-regulation of some or all of these genes contributes to v-src-induced changes in mitogenic control or cell morphology. |
