| First Author | Liu S | Year | 2012 |
| Journal | Invest Ophthalmol Vis Sci | Volume | 53 |
| Issue | 10 | Pages | 6254-62 |
| PubMed ID | 22879415 | Mgi Jnum | J:213967 |
| Mgi Id | MGI:5586949 | Doi | 10.1167/iovs.12-9450 |
| Citation | Liu S, et al. (2012) Tracking retinal microgliosis in models of retinal ganglion cell damage. Invest Ophthalmol Vis Sci 53(10):6254-62 |
| abstractText | PURPOSE: To investigate the longitudinal profiles of microgliosis after optic nerve injury induced by optic nerve crush and acute elevation of intraocular pressure (IOP). METHODS: A confocal scanning laser ophthalmoscope was used to image the retinal microglia of the CX3CR1(GFP/+) transgenic mice in vivo at baseline, 3 days and then weekly for 4 weeks after optic nerve crush (n = 3), and after elevating the IOP to 110 mm Hg for 30 (n = 3) or 60 (n = 3) minutes. RESULTS: After optic nerve crush, the density of microglia increased by 2.43 +/- 0.19-fold at week 1 and then gradually declined with 2.04 +/- 0.24-, 1.69 +/- 0.25-, and 1.29 +/- 0.11-fold increases at week 2, 3, and 4, respectively. Microgliosis followed a similar pattern after acute IOP elevation and the increase in microglia was associated with the duration of IOP elevation. There were 1.35 +/- 0.17- and 2.03 +/- 0.08-fold increases in microglia at week 1, and 1.15 +/- 0.11- and 1.11 +/- 0.10-fold increases at week 4, after 30 and 60 minutes of acute IOP elevation, respectively. The morphology of microglia changed from ramified to ameboid form in 1 week, and then returned to ramified form in the subsequent weeks. There was a significant negative association between the number of surviving retinal ganglion cells (RGCs) and the extent of microgliosis during the follow-up period (R(2) = 0.72, P = 0.004). CONCLUSIONS: Longitudinal in vivo imaging of the retinal microglia can provide an effective approach to study microgliosis and its association with RGC degeneration. |
