Primary Identifier | MGI:3051541 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Atp2b1 |
Transmission | Germline | Strain of Origin | 129X1/SvJ |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Codons 466-529 in exon 10, including the catalytic phosphorylation site (aspartic acid 475), were replaced with a neomycin resistance gene. Northern blot indicated no difference in levels of mRNA between wild-type and heterozygous mice. RT-PCR confirmed that the exon 9 was spliced to exon 11, producing the transcript of the expected length. |