| First Author | Losman JA | Year | 1993 |
| Journal | Arthritis Rheum | Volume | 36 |
| Issue | 4 | Pages | 552-60 |
| PubMed ID | 8457229 | Mgi Jnum | J:12234 |
| Mgi Id | MGI:60484 | Doi | 10.1002/art.1780360417 |
| Citation | Losman JA, et al. (1993) Nucleosome-specific antibody from an autoimmune MRL/Mp-lpr/lpr mouse. Arthritis Rheum 36(4):552-60 |
| abstractText | OBJECTIVE. To characterize the binding properties and variable-region sequences of LG4-1, a monoclonal antibody from an autoimmune MRL/Mp-lpr/lpr mouse that reacts specifically with nucleosome core particles and represents a new antinuclear antibody specificity. METHODS. The reactivity of the antibody against various nuclear substrates was determined using an enzymatic immunoassay, and the variable-region genes were sequenced from messenger RNA, using the dideoxy chain termination method. RESULTS. LG4-1 was found to react with nucleosome core particles but not with individual histones and DNA, or with various histone-histone and histone-DNA complexes. It was demonstrated that this antibody is encoded by a combination of variable-region genes and gene segments that have undergone few somatic mutations. CONCLUSION. The nucleosome core particle expresses a unique conformational autoepitope(s) resulting from the ordered association of histones and DNA. |
