| First Author | Klinman DM | Year | 1993 |
| Journal | Arthritis Rheum | Volume | 36 |
| Issue | 4 | Pages | 561-8 |
| PubMed ID | 8457230 | Mgi Jnum | J:11845 |
| Mgi Id | MGI:60115 | Doi | 10.1002/art.1780360418 |
| Citation | Klinman DM, et al. (1993) VH family utilization by IgG anti-DNA-secreting lymphocytes derived from autoimmune MRL-lpr/lpr mice [see comments]. Arthritis Rheum 36(4):561-8 |
| abstractText | OBJECTIVE. To evaluate the heterogeneity of the IgG anti-DNA autoantibody response of MRL/lpr mice. METHODS. B cell clones were grown on nitrocellulose membranes. Those producing IgG anti-DNA antibodies were identified by a modified enzyme-linked immunospot assay, and their utilization of IgVH genes was determined by hybridization. RESULTS. Four hundred sixty-eight IgG anti-DNA-secreting colonies were derived from 9 autoimmune MRL/lpr mice. Individual VH families contributed to anti-DNA production at a frequency roughly proportional to their representation in the expressed repertoire (except for the more frequent use of VH 7183 and less frequent use of VH 36-60). In individual mice, anti-DNA antibodies were encoded by 2-5 different IgVH families, with no single family constituting more than 27-65% of any animal's anti-DNA response. CONCLUSION. The IgG anti-DNA response of individual MRL/lpr mice is oligoclonal. |
