| Primary Identifier | MGI:7623879 | Allele Type | Endonuclease-mediated |
| Gene | Tet2 | Transmission | Germline |
| Strain of Origin | (C57BL/6 x 129S4/SvJae)F1 | Is Recombinase | false |
| Is Wild Type | false |
| molecularNote | Histidine codon 1295 (CAC) in exon 9 was changed to tyrosine (TAC) (ENSMUSP00000096203:p.H1295Y) and aspartic acid codon 1297 (GAC) was changed to alanine (GCC) (ENSMUSP00000096203:p.D1297A) using an sgRNA and an ssODN template with CRISPR/Cas9 technology. These mutations are in the Fe2+ binding pocket of the encoded protein and cause the loss of its catalytic activity. |
