| First Author | Nie GY | Year | 2000 |
| Journal | Mol Hum Reprod | Volume | 6 |
| Issue | 12 | Pages | 1131-9 |
| PubMed ID | 11101696 | Mgi Jnum | J:66654 |
| Mgi Id | MGI:1928804 | Doi | 10.1093/molehr/6.12.1131 |
| Citation | Nie GY, et al. (2000) Uterine expression of alternatively spliced mRNAs of mouse splicing factor SC35 during early pregnancy. Mol Hum Reprod 6(12):1131-9 |
| abstractText | RNA differential display was applied to identify genes critical for the establishment of pregnancy in the mouse. One of the gene fragments identified was homologous to human SC35 splicing factor; the mouse counterpart had not then been cloned. To obtain the full cDNA sequence of the mouse gene, a cDNA library was screened and four positive clones were fully analysed. Sequencing analysis indicated that we had cloned alternatively spliced mRNA species of mouse SC35 splicing factor. A map of splicing structure for this gene's pre-mRNA was then proposed and region-specific mRNA species were tested on Northern blots. This analysis indicated that the overall expression level of SC35 mRNA was much higher in implantation sites than in inter-implantation sites in the mouse uterus during early pregnancy. The expression of alternatively spliced mRNAs for SC35 was differently regulated both during early pregnancy and by steroid hormones. Embryo-derived factors were also implicated in the up-regulation of SC35 mRNA at implantation sites. These results demonstrate, for the first time, that an essential splicing factor is regulated in a complex manner during implantation in the mouse uterus. Hence, its correct regulation could be important for the success of pregnancy. |
