| First Author | Floss T | Year | 1996 |
| Journal | Dev Biol | Volume | 174 |
| Issue | 1 | Pages | 140-7 |
| PubMed ID | 8626014 | Mgi Jnum | J:31636 |
| Mgi Id | MGI:79122 | Doi | 10.1006/dbio.1996.0058 |
| Citation | Floss T, et al. (1996) Myf-5(m1)/Myf-6(m1) compound heterozygous mouse mutants down-regulate Myf-5 expression and exert rib defects: evidence for long-range cis effects on Myf-5 transcription. Dev Biol 174(1):140-7 |
| abstractText | Myf-6 and Myf-5, two members of the family of muscle- specific regulatory genes, are located less than 10 kb apart in the mouse and human genomes. We have shown recently that homozygous mutant mice carrying a pgk-neo- cassette in the first exon of the Myf-6 gene display minor alterations of skeletal musculature but develop a severe rib defect, most likely due to a drastic down-regulation of Myf-5 expression. The mechanism by which the Myf-6 mutation affects the Myf-5 gene is unknown. In order to determine whether Myf-5 transcription is inhibited by the Myf-6 mutation in cis or in trans, we generated compound heterozygous mice carrying inactivated Myf-5 and Myf-6 alleles on different chromosomes. Here, we demonstrate that double-heterozygous mutants exhibit truncated ribs and severe depression of Myf-5 transcription, a phenotype similar to the previously described homozygous Myf-6 mutant mice. These results indicate that the Myf-6 mutation inhibits Myf-5 gene expression by a long-range cis effect. (C) 1996 Academic Press, Inc. |
