Primary Identifier | MGI:3815117 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Sptlc2 |
Transmission | Germline | Strain of Origin | Not Specified |
Is Recombinase | false | Is Wild Type | false |
molecularNote | Exon 1, which contains the ATG start codon, was replaced with a neomycin resistance cassette. Southern blotting confirmed correct targeting of ES cells. Immunoblot analysis on heterozygote liver extracts indicated protein expression was reduced by more than half, confirming gene inactivation. |