Primary Identifier | MGI:6759774 | Allele Type | Targeted |
Attribute String | Conditional ready, Inserted expressed sequence, Reporter | Gene | Mapk1 |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | The targeting construct inserted a floxed IRES-beta-geo fusion cassette, floxed exons 2, a partial cDNA starting from exon 3 to the end of the polyadenylation signal sequence encoding the amino acid substitution of aspartic acid with arginine at position 319 (D319N), and a neomycin resistance cassette. After removing the gene trap cassette by the cre recombinase, the allele expresses a modified protein product with the amino acid substitution. |