| First Author | Demoulin JB | Year | 1999 |
| Journal | Eur Cytokine Netw | Volume | 10 |
| Issue | 1 | Pages | 49-56 |
| PubMed ID | 10210773 | Mgi Jnum | J:55985 |
| Mgi Id | MGI:1339850 | Citation | Demoulin JB, et al. (1999) Ly-6A/E induction by interleukin-6 and interleukin-9 in T cells. Eur Cytokine Netw 10(1):49-56 |
| abstractText | Interleukin-6 (IL-6) is active in the early steps of T cell activation and confers IL-2 responsiveness. In this work, we used EL4 T lymphoma to identify IL-6 target genes in T cells. By differential screening of a cDNA library, we found that IL-6 induced the expression of Ly-6A/E, a GPI-anchored cell surface protein reported to be a regulator of T cell activation. In addition to IL-6, IL-9 and IFN-gamma induced Ly-6A/E expression in EL4 and BW5147 cells. We showed that both IL-6 and IL-9 mediated the transcriptional activation of Ly-6A/E through a GAS element in the Ly-6A/E promoter, which was able to bind STAT1 and STAT3, transcription factors activated by these cytokines. IL-6 had a similar effect in freshly isolated normal T cells, and dramatically increased their proliferation upon Ly-6A/E stimulation. Taken together, our data suggest that Ly-6A/E induction takes part in the T cell activation program initiated by IL-6. |
