| First Author | Meredith SA | Year | 1978 |
| Journal | Genetics | Volume | 90 |
| Issue | 4 | Pages | 725-34 |
| PubMed ID | 744474 | Mgi Jnum | J:6098 |
| Mgi Id | MGI:54575 | Doi | 10.1093/genetics/90.4.725 |
| Citation | Meredith SA, et al. (1978) Apparent trans control of murine beta-glucuronidase synthesis by a temporal genetic element. Genetics 90(4):725-34 |
| abstractText | A difference in the heat-inactivation kinetics between the beta-glucuronidases of C3HeB/FeJ and C57B1/6J mice was utilized to assess the mode of action of a temporal genetic element in controlling the expression of the beta-glucuronidase structural gene Gus. The heat-inactivation kinetics of liver and kidney beta-glucuronidase from F1 C3HeB/FeJ X C57B1/6J animals were intermediate with respect to the parental enzyme patterns, suggesting that equal concentrations of the two allelic products were present in beta-glucuronidase tetramers of F1 progeny. beta-glucuronidase heteropolymers assembled in vivo under conditions where equal concentrations of the two structural alleles of the enzyme were known to be present also exhibited intermediate heat-inactivation kinetics. These observations are consistent with a trans mode of action of a genetic element that controls the rate of murine beta-glucuronidase synthesis. |
