Primary Identifier | MGI:6273225 | Allele Type | Targeted |
Attribute String | Humanized sequence, Null/knockout | Gene | Scn2a |
Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A loxP site and FRT flanked neo cassette were inserted in intron 1 and an arginine to stop (p.Arg102*; c.304 CGG>TGA) mutation was inserted in exon 2 via homologous recombination. Flp-mediated recombination removed the neo cassette. Western blot analysis indicated lack of expression in whole brain lysates from homozygous mice. |