| Primary Identifier | MGI:3810287 | Allele Type | Targeted |
| Attribute String | Null/knockout | Gene | Del(7Or2h1-Rr320)1Mkg |
| Transmission | Germline | Strain of Origin | 129P2/OlaHsd |
| Is Recombinase | false | Is Wild Type | false |
| description | Male ES cells, deltaLCR-D/S-1, were generated from male mice on a 129 background that were heterozygous for the Hbb-artm1.1Mkg allele, carried two different variants of the Hbb genes (HbbD/HbbS) and were hemizygous for the null Hprtb-m3 allele. |
| molecularNote | ES cells carrying the Rr320tm1.1Mkg allele, which has most of the Rr320 (Hbb-ar) region replaced with a loxP site, were targeted in cis with a construct that replaced the upstream HS-62.5 element with a loxP site flanked neomycin selection cassette. This substitution removed nucleotides -63238 through -59669, relative to the start site of the Hbb-y gene. Transient expression of Cre recombinase eliminated the entire genomic sequence between the newly inserted floxed neo cassette and the loxP site present from the Rr320rtm1.1Mkg allele. This deletion removes genes Olfr64, Olf65, Olf66, and almost all of the Hbb-ar region. |
