| First Author | Wong MK | Year | 2000 |
| Journal | Biochem Biophys Res Commun | Volume | 268 |
| Issue | 3 | Pages | 853-9 |
| PubMed ID | 10679295 | Mgi Jnum | J:113874 |
| Mgi Id | MGI:3687786 | Doi | 10.1006/bbrc.2000.2173 |
| Citation | Wong MK, et al. (2000) A non-transmembrane form of Jagged-1 regulates the formation of matrix-dependent chord-like structures. Biochem Biophys Res Commun 268(3):853-9 |
| abstractText | Jagged-Notch interactions regulate a transmembrane ligand-receptor signaling pathway involved in the regulation of cell fate determination as well as myoblast and endothelial cell differentiation. To further examine the role of the transmembrane ligand, Jagged-1, in the regulation of cell differentiation, we stably transfected NIH 3T3 cells with a truncated form of Jagged(J)-1, which results in the secretion of a soluble(s) form of the protein. Comparison of gene expression by serial analysis demonstrated that among the 227 transcripts differentially regulated in the sJ-1 transfectants, the expression of the pro-alpha-2(I) collagen transcript and pro-alpha-1(I) collagen translation product was predominantly repressed in sJ-1 transfectants. When plated on extracellular matrices, sJ-1 transfectants formed prominent chord-like structures on type I collagen but not on fibrin, fibronectin, or vitronectin. While the sJ-1 transfectants exhibited growth kinetics similar to control cells and were unable to grow in soft agar, the cells were less sensitive to contact inhibition of growth in vitro and sJ-1 allografts formed tissue masses in nude mice after a prolonged latency period and exhibited an abundance of host-derived microvascular endothelial cells. These data suggest that J-1 may be able to modulate, in a matrix-dependent manner, the organization of cell to cell interactions including its ability to promote the development of chord-like structures. |
