| First Author | Yamauchi N | Year | 1994 |
| Journal | Mol Cell Biol | Volume | 14 |
| Issue | 10 | Pages | 6655-62 |
| PubMed ID | 7935384 | Mgi Jnum | J:20915 |
| Mgi Id | MGI:68975 | Doi | 10.1128/mcb.14.10.6655 |
| Citation | Yamauchi N, et al. (1994) The Ras/Raf signaling pathway is required for progression of mouse embryos through the two-cell stage. Mol Cell Biol 14(10):6655-62 |
| abstractText | We have used microinjection of antisense oligonucleotides, monoclonal antibody, and the dominant negative Ras N-17 mutant to interfere with Ras expression and function in mouse oocytes and early embryos. Microinjection of either ras antisense oligonucleotides or anti-Ras monoclonal antibody Y13-259 did not affect normal progression of oocytes through meiosis and arrest at metaphase II. However, microinjection of fertilized eggs with constructs expressing Ras N-17 inhibited subsequent development through the two-cell stage. The inhibitory effect of Ras N-17 was overcome by simultaneous injection of a plasmid expressing an active raf oncogene, indicating that it resulted from interference with the Ras/Raf signaling pathway. In contrast to the inhibition of two-cell embryo development resulting from microinjection of pronuclear stage eggs, microinjection of late two-cell embryos with Ras N-17 expression constructs did not affect subsequent cleavages and development to morulae and blastocysts. It thus appears that the Ras/Raf signaling pathway, presumably activated by autocrine growth factor stimulation, is specifically required at the two-cell stage, which is the time of transition between maternal and embryonic gene expression in mouse embryos. |
