| First Author | Ruiz-Vela A | Year | 2007 |
| Journal | FEBS Lett | Volume | 581 |
| Issue | 18 | Pages | 3422-8 |
| PubMed ID | 17618626 | Mgi Jnum | J:123727 |
| Mgi Id | MGI:3719335 | Doi | 10.1016/j.febslet.2007.06.049 |
| Citation | Ruiz-Vela A, et al. (2007) Proapoptotic histone H1.2 induces CASP-3 and -7 activation by forming a protein complex with CYT c, APAF-1 and CASP-9. FEBS Lett 581(18):3422-8 |
| abstractText | Cytochrome c (CYT c) is a protein that employs the caspase recruitment domain (CARD)-containing proteins APAF-1 and CASP-9 to activate effectors CASP-3 and -7. By using affinity labeling techniques and mass spectrometry analysis, we show that histone H1.2 is a regulator of caspases upon UV irradiation. We demonstrated that histone H1.2 forms a protein complex with APAF-1, CASP-9 and CYT c upon UV irradiation. In cell-free systems, we show that histone H1.2 triggers activation of CASP-3 and -7 via APAF-1 and CASP-9. We therefore conclude that upon DNA damage histone H1.2 acts as a positive regulator of apoptosome formation. |
