| First Author | Dhakal M | Year | 2014 |
| Journal | Eur J Immunol | Volume | 44 |
| Issue | 3 | Pages | 842-55 |
| PubMed ID | 24281978 | Mgi Jnum | J:209079 |
| Mgi Id | MGI:5565641 | Doi | 10.1002/eji.201343755 |
| Citation | Dhakal M, et al. (2014) IL-13Ralpha1 is a surface marker for M2 macrophages influencing their differentiation and function. Eur J Immunol 44(3):842-55 |
| abstractText | In this study, we examined the role IL-13 receptor alpha 1 (IL-13Ralpha1) plays in macrophage differentiation and function. The findings indicate that IL-13Ralpha1 is expressed on the M2 but not on the M1 subset of macrophages and specifically heterodimerizes with the IL-4Ralpha chain to form a type II receptor, which controls the differentiation and function of these cells. Indeed, BM cells from IL-13Ralpha1(+/+) and IL-13Ralpha1(-/-) mice yield equivalent numbers of macrophages when cultured under M2 polarizing conditions. However, IL-13Ralpha1(-/-) BM cells yield a much higher number of macrophages than IL-13Ralpha1(+/+) BM cells when the differentiation is carried out under M1-polarizing conditions. Further analyses indicated that macrophages that express IL-13Ralpha1 also display surface markers associated with an M2 phenotype. In addition, the IL-13Ralpha1(+) macrophages were highly efficient in phagocytizing zymosan bioparticles both in vitro and in vivo, and supported differentiation of naive T cells to a Th2 phenotype. Finally, when stimulated by IL-13, a cytokine that uses the heteroreceptor, the cells were able to phosphorylate STAT6 efficiently. These previously unrecognized findings indicate that IL-13Ralpha1 serves as a marker for M2 macrophages and the resulting heteroreceptor influences both their differentiation and function. |
