| First Author | Kallenbach S | Year | 1993 |
| Journal | Int Immunol | Volume | 5 |
| Issue | 2 | Pages | 231-2 |
| PubMed ID | 8452820 | Mgi Jnum | J:12867 |
| Mgi Id | MGI:61084 | Doi | 10.1093/intimm/5.2.231 |
| Citation | Kallenbach S, et al. (1993) Rag-1: a topoisomerase?. Int Immunol 5(2):231-2 |
| abstractText | Recombination activating genes Rag-1 and Rag-2 were isolated on the basis of their ability to confer V(D)J recombination activity when co-expressed in fibroblasts. The mode of action of the confer V(D)J recombination activity when co-expressed in fibroblasts. The mode of action of the products of these genes is not known. Based on sequence comparison data, it was suggested that Rag-1 protein could act like a topoisomerase and that tyrosine in position 998 could be the active site tyrosine. We tested this hypothesis by introducing a point mutation on the Rag-1 cDNA, transforming the tyrosine codon into a phenylalanine codon. We show that the mutation has no effect on site specific recombination implying that Tyr-998 is not essential for the recombination reaction. |
