| First Author | Grenningloh R | Year | 2008 |
| Journal | Eur J Immunol | Volume | 38 |
| Issue | 6 | Pages | 1700-5 |
| PubMed ID | 18465773 | Mgi Jnum | J:136194 |
| Mgi Id | MGI:3795618 | Doi | 10.1002/eji.200738112 |
| Citation | Grenningloh R, et al. (2008) Role of Ets-1 phosphorylation in the effector function of Th cells. Eur J Immunol 38(6):1700-5 |
| abstractText | The transcription factor Ets-1 critically regulates differentiation and function of T helper (Th) cells. In vitro studies have demonstrated that DNA binding and transcriptional activity of Ets-1 are regulated by phosphorylation. Depending on the site of phosphorylation, Ets-1 function can either be increased or inhibited. In addition, a splice variant lacking several inhibitory phosphorylation sites has been identified, raising the possibility that this splice variant may function differently from the full-length Ets-1. However, it is unclear how the activating and inhibitory phosphorylation events of Ets-1 are coordinated during Th cell activation. Furthermore, the biological consequences of Ets-1 phosphorylation and alternative splicing in regulating the function of Th cells are unknown. We report here that both activating and inhibitory phosphorylation events of Ets-1 occur simultaneously and independently of each other during Th cell activation. We further demonstrate that the effect of Ets-1 phosphorylation is very modest and that full-length Ets-1 and its splice variant are functionally interchangeable in the regulation of cytokine production in Th cells.Supporting Information for this article is available at www.wiley-vch.de/contents/jc_2040/2008/38112_s.pdf. |
