| First Author | Wagner C | Year | 1998 |
| Journal | J Clin Invest | Volume | 102 |
| Issue | 8 | Pages | 1576-82 |
| PubMed ID | 9788971 | Mgi Jnum | J:115239 |
| Mgi Id | MGI:3691170 | Doi | 10.1172/JCI4044 |
| Citation | Wagner C, et al. (1998) Role of cGMP-kinase II in the control of renin secretion and renin expression. J Clin Invest 102(8):1576-82 |
| abstractText | To investigate the roles of the cGMP-dependent protein kinases (cGKs) in the control of the renin system, we studied the regulation of renin in cGKI- or cGKII-deficient mice in vivo and in vitro. Renal renin mRNA levels both under stimulatory (low-salt diet plus ramipril) and inhibitory (high-salt diet) conditions were not different between wild-type and cGKI-/- mice, but were significantly elevated in cGKII-/- mice under all experimental conditions. In primary cultures of renal juxtaglomerular cells (JG) established from wild-type, cGKI-/-, and cGKII-/- mice, the adenylate cyclase activator forskolin stimulated renin secretion similarly in all genotypes tested. 8-bromo-cGMP attenuated basal and forskolin-stimulated renin secretion in cultures from wild-type and cGKI-/-, but had no effect in cells isolated from cGKII-/- mice. Activation of cGKs by 8-bromo-cGMP decreased renin secretion from the isolated perfused rat kidney, independent of prestimulation by beta-adrenoreceptor activation, macula densa inhibition, reduced perfusion pressure, or by a nominally calcium-free perfusate. Taken together, these findings suggest that activation of cGKII has a general inhibitory effect on renin secretion from renal JG cells. |
