Primary Identifier | MGI:6437883 | Allele Type | Endonuclease-mediated |
Attribute String | Null/knockout | Gene | Ryr1 |
Strain of Origin | C57BL/6NCrl | Is Recombinase | false |
Is Wild Type | false |
molecularNote | CRISPR/Cas9 endonuclease-mediated genome editing introduced an indel comprised of a 16 bp (Chr7:29013738 to 29013752) deletion in exon 96 of the gene. Sequencing N1 pups revealed the repair template was not incorporated into this allele, which resulted from non-homologous end-joining repair of the double strand break. |