| First Author | Kojima H | Year | 1994 |
| Journal | Immunity | Volume | 1 |
| Issue | 5 | Pages | 357-64 |
| PubMed ID | 7533644 | Mgi Jnum | J:25012 |
| Mgi Id | MGI:72727 | Doi | 10.1016/1074-7613(94)90066-3 |
| Citation | Kojima H, et al. (1994) Two distinct pathways of specific killing revealed by perforin mutant cytotoxic T lymphocytes. Immunity 1(5):357-64 |
| abstractText | To study the contribution of putative perforin-independent mechanism in the antigen-specific target destruction by cytotoxic T lymphocytes CD8+ CTL lines were established from spleen cells of chimeric mice produced by injecting perforin (-/-) embryonic stem cells into blastocysts of RAG-2(-/-) mice. When tested on normal concanavalin A blasts, these perforin-deficient cytotoxic T lymphocyte lines were found to be capable of inducing antigen-specific target cell lysis accompanied by DNA degradation. In contrast, with target cells carrying a mutation in Fas molecule, perforin-independent cytotoxicity was not detectable. These data not only confirmed the primary role of perforin but simultaneously revealed a major contribution of a perforin-independent Fas-mediated pathway in antigen-specific cytolysis. |
