Primary Identifier | MGI:2388132 | Allele Type | Targeted |
Attribute String | Null/knockout | Gene | Ikzf3 |
Transmission | Germline | Strain of Origin | 129S4/SvJae |
Is Recombinase | false | Is Wild Type | false |
molecularNote | A 0.35 kb region of exon 7 including the 5' splice acceptor site was replaced with a PGK-neo cassette via homologous recombination. Gene expression was absent in homozygous mutant animals as demonstrated by Northern blot analysis of spleen, bone marrow, and thymus samples using probes to exons 1-6 and exon 7. Immunohistochemical analysis of spleen sections from 4 week old homozygous mutant mice did not detect protein product. |