| First Author | Rinaldi T | Year | 2003 |
| Journal | Gene | Volume | 303 |
| Pages | 63-8 | PubMed ID | 12559567 |
| Mgi Jnum | J:81883 | Mgi Id | MGI:2450189 |
| Doi | 10.1016/s0378-1119(02)01154-x | Citation | Rinaldi T, et al. (2003) Nucleo-mitochondrial interactions in Saccharomyces cerevisiae: characterization of a nuclear gene suppressing a defect in mitochondrial tRNA(Asp) processing. Gene 303(1-2):63-8 |
| abstractText | We utilized the heat-sensitive mutant strain (Ts932), bearing a mutation at position 61 in the mitochondrial tRNA(Asp) gene, to identify nuclear genes involved in tRNA biogenesis; this mutant is defective in 3'-end processing and consequently in the production of mature mitochondrial tRNA(Asp). We transformed this strain with a yeast nuclear library and we isolated among other suppressors, an unknown, non-essential gene (called SMM1, corresponding to open reading frame YNR015w), which restored the growth on glycerol and a normal amount of processed tRNA(Asp) in the mutant. The gene contains a domain highly conserved in evolution from bacteria to human and its product has been recently shown to have dihydrouridine synthase activity. |
