| Type | MGI:General | Description | loading sperm populations with Cal520-AM (an Ca2+ dye) followed by stimulation with K8.6, 8-Br-cAMP, NH4Cl, or with the Ca2+-ionophore ionomycin fails to evoke a Ca2+ influx in sperm, unlike in similarly treated wild-type and heterozygous sperm fluorescence microscopy showed that sperm loaded with Cal520-AM show no fluorescence in the midpiece, unlike similarly loaded heterozygous sperm, suggesting that severe plasma membrane defects may prevent dye loading |
