| First Author | Hayashi H | Year | 2002 |
| Journal | Neuroreport | Volume | 13 |
| Issue | 4 | Pages | 383-6 |
| PubMed ID | 11930145 | Mgi Jnum | J:103515 |
| Mgi Id | MGI:3610249 | Doi | 10.1097/00001756-200203250-00004 |
| Citation | Hayashi H, et al. (2002) Cholesterol is increased in the exofacial leaflet of synaptic plasma membranes of human apolipoprotein E4 knock-in mice. Neuroreport 13(4):383-6 |
| abstractText | Inheritance of the apolipoprotein (apoE) epsilon4 allele is a risk factor for developing Alzheimer's disease (AD). The purpose of the present study was to determine effects of apoE-isoforms on the transbilayer distribution of cholesterol in synaptic plasma membranes (SPM) using mice expressing human apoE3 and apoE4. Total SPM cholesterol levels did not differ among the wild-type and apoE3 and apoE4 knock-in mice. However, a striking difference was observed in the transbilayer distribution of SPM cholesterol. ApoE4 knock-in mice showed an approximately 2-fold increase in exofacial leaflet cholesterol compared with apoE3 knock-in mice and wild-type mice. The results of this study suggest that pathogenic effects of apoE4 on AD development could be closely linked to alteration of cholesterol distribution in SPM. |
